Human testis in organotypic culture: application for basic or clinical research
Identifieur interne : 001647 ( Main/Exploration ); précédent : 001646; suivant : 001648Human testis in organotypic culture: application for basic or clinical research
Auteurs : V. Roulet [France] ; H. Denis [France] ; C. Staub [France] ; A. Le Tortorec [France] ; B. Delaleu ; A. P. Satie [France] ; J. J. Patard [France] ; B. Je Gou [France] ; N. Dejucq-Rainsford [France]Source :
- Human Reproduction [ 0268-1161 ] ; 2006-06.
English descriptors
- KwdEn :
Abstract
BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2′-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue.
Url:
DOI: 10.1093/humrep/del018
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000E45
- to stream Istex, to step Curation: 000E36
- to stream Istex, to step Checkpoint: 001093
- to stream Main, to step Merge: 001655
- to stream Main, to step Curation: 001647
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human testis in organotypic culture: application for basic or clinical research</title><author><name sortKey="Roulet, V" sort="Roulet, V" uniqKey="Roulet V" first="V." last="Roulet">V. Roulet</name></author><author><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name></author><author><name sortKey="Staub, C" sort="Staub, C" uniqKey="Staub C" first="C." last="Staub">C. Staub</name></author><author><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name></author><author><name sortKey="Delaleu, B" sort="Delaleu, B" uniqKey="Delaleu B" first="B." last="Delaleu">B. Delaleu</name></author><author><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A. P." last="Satie">A. P. Satie</name></author><author><name sortKey="Patard, J J" sort="Patard, J J" uniqKey="Patard J" first="J. J." last="Patard">J. J. Patard</name></author><author><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name></author><author><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:84604146EBCF09F64ECBACBCBB13D22C8B28D26E</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1093/humrep/del018</idno><idno type="url">https://api.istex.fr/document/84604146EBCF09F64ECBACBCBB13D22C8B28D26E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E45</idno><idno type="wicri:Area/Istex/Curation">000E36</idno><idno type="wicri:Area/Istex/Checkpoint">001093</idno><idno type="wicri:doubleKey">0268-1161:2006:Roulet V:human:testis:in</idno><idno type="wicri:Area/Main/Merge">001655</idno><idno type="wicri:Area/Main/Curation">001647</idno><idno type="wicri:Area/Main/Exploration">001647</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Human testis in organotypic culture: application for basic or clinical research</title><author><name sortKey="Roulet, V" sort="Roulet, V" uniqKey="Roulet V" first="V." last="Roulet">V. Roulet</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Staub, C" sort="Staub, C" uniqKey="Staub C" first="C." last="Staub">C. Staub</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Delaleu, B" sort="Delaleu, B" uniqKey="Delaleu B" first="B." last="Delaleu">B. Delaleu</name><affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A. P." last="Satie">A. P. Satie</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Patard, J J" sort="Patard, J J" uniqKey="Patard J" first="J. J." last="Patard">J. J. Patard</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>6To whom correspondence should be addressed at: INSERM U625-GERHM, Campus de Beaulieu, 35042 Rennes Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author><author><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>INSERM, U625, GERHM, Campus de Beaulieu, Université de Rennes 1, IFR 140, Rennes, Service de microscopie électronique, INRA PRC, Tours and Service d’Urologie, CHUR Pontchaillou, Rennes</wicri:regionArea><placeName><settlement type="city">Rennes</settlement></placeName><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>7To whom correspondence should be addressed at: INSERM U625-GERHM, Campus de Beaulieu, 35042 Rennes Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation wicri:level="4"><country>France</country><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName><orgName type="university">Université de Rennes 1</orgName><orgName type="institution" wicri:auto="newGroup">Université européenne de Bretagne</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Human Reproduction</title><title level="j" type="abbrev">Hum. Reprod.</title><idno type="ISSN">0268-1161</idno><idno type="eISSN">1460-2350</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2006-06">2006-06</date><biblScope unit="volume">21</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1564">1564</biblScope><biblScope unit="page" to="1575">1575</biblScope></imprint><idno type="ISSN">0268-1161</idno></series><idno type="istex">84604146EBCF09F64ECBACBCBB13D22C8B28D26E</idno><idno type="DOI">10.1093/humrep/del018</idno><idno type="local">018</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0268-1161</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>human</term><term>organotypic culture</term><term>testis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2′-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement><orgName><li>Université de Rennes 1</li><li>Université européenne de Bretagne</li></orgName></list><tree><noCountry><name sortKey="Delaleu, B" sort="Delaleu, B" uniqKey="Delaleu B" first="B." last="Delaleu">B. Delaleu</name></noCountry><country name="France"><noRegion><name sortKey="Roulet, V" sort="Roulet, V" uniqKey="Roulet V" first="V." last="Roulet">V. Roulet</name></noRegion><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Dejucq Rainsford, N" sort="Dejucq Rainsford, N" uniqKey="Dejucq Rainsford N" first="N." last="Dejucq-Rainsford">N. Dejucq-Rainsford</name><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name><name sortKey="Denis, H" sort="Denis, H" uniqKey="Denis H" first="H." last="Denis">H. Denis</name><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name><name sortKey="Je Gou, B" sort="Je Gou, B" uniqKey="Je Gou B" first="B." last="Je Gou">B. Je Gou</name><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name><name sortKey="Le Tortorec, A" sort="Le Tortorec, A" uniqKey="Le Tortorec A" first="A." last="Le Tortorec">A. Le Tortorec</name><name sortKey="Patard, J J" sort="Patard, J J" uniqKey="Patard J" first="J. J." last="Patard">J. J. Patard</name><name sortKey="Roulet, V" sort="Roulet, V" uniqKey="Roulet V" first="V." last="Roulet">V. Roulet</name><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A. P." last="Satie">A. P. Satie</name><name sortKey="Satie, A P" sort="Satie, A P" uniqKey="Satie A" first="A. P." last="Satie">A. P. Satie</name><name sortKey="Staub, C" sort="Staub, C" uniqKey="Staub C" first="C." last="Staub">C. Staub</name><name sortKey="Staub, C" sort="Staub, C" uniqKey="Staub C" first="C." last="Staub">C. Staub</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001647 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001647 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:84604146EBCF09F64ECBACBCBB13D22C8B28D26E
|texte= Human testis in organotypic culture: application for basic or clinical research
}}
| This area was generated with Dilib version V0.6.25. |  |